发明名称 VERFAHREN ZUM ASSAY VON TROPONIN I UND T UND KOMPLEXEN VON TROPONIN I UND T UND AUSWAHL VON ANTIKÖRPERN ZUR VERWENDUNG IN IMMUNOASSAYS
摘要 Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I an T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed is a system to determine the presence of a troponin form or a group of troponin forms in a sample of whole blood, serum or plasma. Disclosed is a stabilized composition of troponin; the stabilized composition can comprise a stabilized composition of troponin I, wherein the troponin I is oxidized, the troponin I can be unbound or the troponin I can be in a complex. Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays. Also disclosed are antibodies which recognize, unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.
申请公布号 AT281648(T) 申请公布日期 2004.11.15
申请号 AT19960913812T 申请日期 1996.04.18
申请人 BIOSITE DIAGNOSTICS INC. 发明人 BUECHLER, KENNETH, F.;MCPHERSON, PAUL, H.
分类号 G01N33/53;C12P21/08;G01N33/68 主分类号 G01N33/53
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