| 摘要 |
The invention relates to a method for the chemical synthesis of interferon proteins containing one or more cysteine residues, by the native chemical ligation of segments of the full length interferon protein. The segments of the protein are selected based on the position of the cysteine residues in the interferon protein, with the position of the cysteine residue as a potential site of native chemical ligation. The full-length, fully deprotected polypeptide is folded into biologically active interferon protein by oxidation of the cysteine residues. The invention further relates to the production of the individual peptide fragments which act as intermediates in the synthesis of interferon-alpha. The present invention still further relates to groups of such peptide intermediates which can be utilized together to produce interferon-alpha and interferon-alpha-like proteins.
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