ISOLATION OF MMP-1 SYNTHESIS INHIBITOR FROM EUCOMMIA ULMOIDES AND ITS PURIFICATION METHOD, WHEREIN THE MMP-1 SECRETION INHIBITOR IS USEFUL FOR FUNCTIONAL FOODS, COSMETICS AND DRUGS FOR PREVENTING SKIN AGING

摘要

PURPOSE: Provided are an isolation of MMP-1 synthesis inhibitor from Eucommia ulmoides and its purification method by ethanol extract, fractioning, silicagel column chromatography, HPLC and the like. The MMP-1 synthesis inhibitor is useful for functional foods, cosmetics and drugs for preventing skin aging. CONSTITUTION: The MMP-1 synthesis inhibitor is characterized by containing, as an active ingredient, aucubin extracted from Eucommia ulmoides by: extracting Eucommia ulmoides with methanol at room temperature, followed by concentration at 40 deg.C; fractioning the extract with hexane to give hexane fractions; fractioning the remnant with chloroform to give chloroform fractions; and fractioning the remnant with ethylacetate to give ethylacetate fractions; and concentrating the remnant to give methanol fractions.