| 摘要 |
Disclosed is a method for analyzing a nucleic acid mixture by hybridizing a DNA array comprising a complexity-reduced version of said nucleic acid mixture. According to said method, the nucleic acid mixture that is to be analyzed is split by means of at least one BcgI-similar restriction endonuclease, marked, and optionally isolated. A hybridization sample comprising a marked nucleic acid fragment mixture with improved hybridization specificity and fragments that are essentially of the same length can be obtained by means of several methods. A marked nucleic acid fragment mixture which comprises fragments of substantially identical length and is obtained by means of the inventive method is used for hybridization with immobilized nucleic acids, particularly DNA arrays. The invention also relates to a DNA array comprising immobilized oligonucleotides, the sequence of which is at least largely identical with the sequence of nucleic acid fragments that can be liberated from a nucleic acid mixture by means of one or several BcgI-similar restriction endonucleases. |
