CO2-INDEPENDENT GROWTH MEDIUM FOR MAINTENANCE AND PROPAGATION OF CELLS

摘要

For the propagation or maintenance of cells in vitro, a culture medium which does not require CO2 in excess of the concentration found in ambient air is described. The medium contains the fre e base forms of L-arginine, L-cysteine, L-histidine, L-lysine and L-tyrosine; biotin; fatty acids; pyruvate; asparagine; low leve ls of sodium bicarbonate and is buffered with .beta.-glycerophosphate. Te use of the medium to culture tumor cells to test anti-tumor compounds is also disclosed.