发明名称 |
METHOD OF DETECTING HISTAMINE-PRODUCING BACTERIUM AND PRIMER FOR USE IN THE METHOD |
摘要 |
<p>A method of detecting a histamine-producing bacterium; a method of identifying the bacterial strain thereof; and a primer for use therein. In particular, PCR of a test sample is carried out with the use of a primer containing base sequences of SEQ ID NO. 1 and 2, and thus obtained PCR products are electrophoresed to thereby detect a histamine-producing bacterium. The strain of detected histamine-producing bacterium is identified by SSCP analysis. As a result, the histamine-producing bacterium can be detected accurately and easily by the detection method, and identification of a causative bacterium is facilitated by the identification method, thereby enabling speedy clarification of pollution source.</p> |
申请公布号 |
WO2004094632(A1) |
申请公布日期 |
2004.11.04 |
申请号 |
WO2004JP05959 |
申请日期 |
2004.04.23 |
申请人 |
KIMURA, BON;FUJII, TAKEO;TAKAHASHI, HAJIME |
发明人 |
KIMURA, BON;FUJII, TAKEO;TAKAHASHI, HAJIME |
分类号 |
G01N33/483;C12N15/09;C12Q1/04;C12Q1/68;G01N27/447;G01N33/50;(IPC1-7):C12N15/00;G01N27/26 |
主分类号 |
G01N33/483 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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