摘要 |
<p>The present invention provides polynucleotides comprising an RNA sequence comprising a first stretch of 21 consecutive nucleotides and a second stretch of 21 consecutive nucleotides, complementary to the first stretch of 21 consecutive nucleotides, wherein in first stretch of 21 consecutive nucleotides: in the 5' -> 3' direction, the first nucleotide is an A-nucleotide , the second nucleotide is a C-nucleotide and the last nucleotide is a C-nucleotide; and no stretches of four or more consecutive identical nucleotides are present; the total number of G- and C-nucleotides is between 33-71% of the total number of nucleotides and in the 5' -> 3' direction, consecutive nucleotides 3-21 are homologous to a RNA-molecule. Vectors encoding the polynucleotide of the present inventionare also provided. Furthermore libraries of the polynucleotides and vectors encoding and methods to produce vectors and libraries are given.</p> |
申请人 |
GALAPAGOS GENOMICS N.V.;ARTS, GERARDUS, JOHANNES, FRANCISCUS;VAN DER SCHUEREN, JAN;LAMBRECHT, MARK, JACQUES, YVONNE;DJOKIC, KRISTINA;CLASEN, REMKO, JOHANNES;MESIC, EMIR;GRIFFIOEN, SANDRA;BERGS, CAROLINA, JOHANNA, LEONARDA |
发明人 |
ARTS, GERARDUS, JOHANNES, FRANCISCUS;LAMBRECHT, MARK, JACQUES, YVONNE;DJOKIC, KRISTINA;CLASEN, REMKO, JOHANNES;MESIC, EMIR;GRIFFIOEN, SANDRA;BERGS, CAROLINA, JOHANNA, LEONARDA |