PROBES FOR DETECTING AND QUANTIFICATION OF LIPID SECOND MESSENGERS AND A METHOD OF DETECTING AND QUANTIFYING LIPID SECOND MESSENGERS

摘要

It is intended to provide a probe for detecting and quantifying a lipid seco nd messenger characterized by having a polypeptide specifically binding to the lipid second messenger, two chromophores being bonded to both terminals of t he polypeptide via rigid linker sequences and having fluorescent wavelengths differing from each other, and a membrane localized sequence bonded to one o f the chromophores via a rigid linker sequence. Use of this probe makes it possible to quantitatively detect a lipid second messenger, i.e., clarifying the production site and the production time thereof in living cells.