摘要 |
The present invention relates to: a reductase gene for an alpha -substituted- alpha , beta -unsaturated carbonyl compound which contains a DNA sequence encoding an amino acid sequence represented by SEQ ID NO: 20 and an amino acid sequence represented by SEQ ID NO: 21; an enzyme which is a product of the gene; a plasmid and a transformant each containing the gene DNA; and a method of reducing an alpha -substituted- alpha , beta -unsaturated carbonyl compound using the transformant. According to claim the present invention, there is provided an enzyme gene which is useful in producing a corresponding alpha -substituted- alpha , beta -saturated carbonyl compound optically active at the alpha -position by hydrogenating an alpha , beta -carbon double bond of an alpha -substituted carbonyl compound, which is a compound prochiral at the alpha-position, and an enzyme which is a gene product thereof. |