Method for preparation of a N-terminally modified chemotactic factor

摘要

Preparing pyroGlu-MCP-1 (A; MCP = monocyte chemoattractant protein-1) from recombinant Gln-MCP-1 (B) by incubating (B) at 30-80 [deg] C in a buffer, of pH 2-7.5, that contains 10-160 mM salt until at least 90% of MCP-1 is converted to (A). ACTIVITY : Cardiant; Vasotropic; Cerebroprotective. MECHANISM OF ACTION : CCR2 agonist; CCR4 agonist. MCP-1 is an agonist of the beta -chemokine receptors CCR2 and 4, which are involved in activation of monocytes and adhesion of integrins, resulting in bonding of monocytes to endothelial cells. This causes extravasation of monocytes from the vascular system, and thus growth of collateral and/or other arteries.