发明名称 |
METHOD OF PRODUCING A CONCENTRATE OF ANTI-D IMMUNOGLOBULIN GAND PHARMACEUTICAL PREPARATION WHICH CONTAINS IT |
摘要 |
An anti-D immunoglobulin G-preparation is produced from human plasma containing anti-D IgG or a plasma fraction containing an anti-D IgG in which A) the plasma or the plasma fraction, with a pH in the range of pH 3.5 to 6.5 and a conductivity value in the range of 2 to 4 mS/cm, is subjected to an ion exchange chromatography with an adsorbant which has carboxymethyl groups as functional groups, the anti-D IgG being bound to the adsorbant, B) the adsorbant with the bound anti-D IgG is first rinsed with a wash solution at a pH in the range of to 8 and a conductivity value in the range of 2 to 4 mS/cm, and the anti-D IgG is subsequently eluted, and further C) the eluted anti-D IgG at a pH in the range of 6 to 8 and a conductivity value in the range of 2 to 4 mS/cm is treated with an alkaline adsorbant with ion-exchange characteristics in order to bind unde- sired components, and finally the anti-D IgG is concentrated. The non-infectious anti-D concentrate obtained with a high yield possesses a high specific activity of more than 1% anti-D IgG per gram of total IgG.
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申请公布号 |
CA2138921(C) |
申请公布日期 |
2004.11.02 |
申请号 |
CA19942138921 |
申请日期 |
1994.12.22 |
申请人 |
ROTKREUZSTIFTUNG ZENTRALLABORATORIUM BLUTSPENDEDIENST SRK |
发明人 |
HODLER, GERHARD;LERCH, PETER;STUCKI, MARTIN |
分类号 |
A61K9/08;A61K39/395;A61K47/02;A61K47/18;A61K47/26;C07K1/18;C07K16/00;C07K16/06;C07K16/34;(IPC1-7):A61K39/395;C12N7/04 |
主分类号 |
A61K9/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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