Selecting optimal operating conditions for coupling reactions, useful e.g. in preparation of pharmaceuticals, particularly selection of catalysts, includes immunological detection of coupled products

摘要

Method for screening the operating conditions for a coupling reaction between at least two functional groups. Method for screening the operating conditions for a coupling reaction between at least two functional groups comprises: (1) reacting at least two compounds, E1-X1-G1 and E2-X2-G2, in solution and under predetermined operating conditions, with at least one being a candidate operating condition (COC), to produce a compound (Z) of formula E1-X1-G1-G2-X2-E2; (2) determining the concentration of (Z) at a predetermined time (t), by at least one immunological assay, using at least one antibody (AC1) specific for the molecule (M1) of which E1 is the residue; and (3) evaluating the effect of one or more COC on the reaction, from the measured concentration of (Z). G1, G2 : first and second functional groups; X1 and X2 : covalent bonds or linkers; E1 : residue of molecule M1 for which a specific antibody (AC1) is available; E2 : residue of second molecule M2 for which a second antibody (AC2) is available, or a group able to form at least one covalent bond with AC1 in presence of a coupling agent An independent claim is also included for a kit for the new process.