Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie

摘要

A prokaryotic expression vector including an origin of replication, at least one eukaryotic auxotrophy marker gene which encodes an enzyme required for the synthesis of a product necessary for the survival of at auxotrophic prokaryote under the control of a eukaryotic promoter, a foreign gene under the control of a prokaryotic promoter, and one or more transcription terminators.