The present invention relates to 2', 3'-bis-O-aminoacylated tRNAs and their use to enhance the yields of in vitro protein synthesis. Advantageously, the bisaminoacyl-tRNAs are more stable than corresponding mono-aminoacyl-tRNAs against deacylation and are shown herein to function in protein biosynthesis, contributing up to two amino acids to the derived protein.
申请公布号
WO2004035764(A3)
申请公布日期
2004.10.21
申请号
WO2003US33459
申请日期
2003.10.16
申请人
UNIVERSITY OF VIRGINIA PATENT FOUNDATION;HECHT, SIDNEY, M.;WANG, BIXUM;LODDER, MICHIEL