THE METHOD AND DEVICE FOR ACCELERATING MOLECULAR HYBRIDIZATION BY ULTRASONIC WAVE

摘要

The present method and device is for accelerating molecular hybridization by ultrasonic wave. A prepared hybridization reaction system is placed in the path of ultrasonic wave generated by an ultrasonic generator. High frequency vibration and irradiating pressure of ultrasonic wave result in effective agitation and flow of liquid medium. The flow of liquid medium then carries reaction molecular to move so as to speed up the hybridization reaction. Advantages of the invention include increasing the intensity of hybridization signal significantly so that a complete reaction can be obtained and time can be saved; and improving consistency of hybridization reaction at different locations on the surface of solid medium, uniformity of hybridization and idiosyncrasy of hybridization reaction. Main parts of the device are an ultrasonic resonance element and the corresponding resonance electrocircuit. The device has simple structure with low cost and can be operated easily and automatically. It can be applied to various hybridization reaction systems, such as Southern, Northern and genetic chips.