| 摘要 |
<p>Disclosed is a tissue modulation device comprising an upper surface and a lower surface, wherein the upper surface comprises a recessed region adjacent to a raised region, wherein the device is optically transparent in at least one of the recessed region or the raised region, wherein application of a first portion of a tissue to the raised region depresses the first portion of the tissue relative to a second portion of the tissue that is in apposition to the recessed region, and wherein the optically transparent region of the device is curved at the lower surface to substantially reduce backscattered light in a light path traveling through the optically transparent region to a light collection system. Also disclosed is a method of noninvasive spectroscopic measurement of an analyte in a subject. The method comprises applying tissue of the subject to a tissue modulation device comprising a recessed region adjacent to a raised region so that the raised region depresses a first portion of tissue relative to a second portion of tissue in apposition to the recessed region; irradiating the tissue in a blood-replete state with electromagnetic radiation having an excitation wavelength; collecting the spectra emitted by the tissue in the blood-replete state; irradiating the tissue in a blood-depleted state with electromagnetic radiation having an excitation wavelength; collecting the spectra emitted by the tissue in the blood-depleted state; and analyzing the collected spectra to determine a concentration of analyte present in the tissue, wherein the analyzing comprises determining the difference between the spectra collected in the blood-replete and blood-depleted states.</p> |
