发明名称 |
SCHUTZGRUPPEN UND IHRE VERWENDUNG IN EINEM VERFAHREN ZUR OLIGONUKLEOTID-SYNTHESE |
摘要 |
Phosphoramidite oligonucleotide synthesis is facilitated by the use of fluoride-labile 5' silyl protecting groups. RNA synthesis is improved by the use of 2' orthoester protecting groups. Reactions are conducted on a solid phase support, and acidic deprotection conditions are avoided, as is the necessity of oxidizing the phosphite linkage between each coupling reaction. |
申请公布号 |
AT277067(T) |
申请公布日期 |
2004.10.15 |
申请号 |
AT19960919324T |
申请日期 |
1996.06.07 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF COLORADO |
发明人 |
SCARINGE, STEPHEN;CARUTHERS, MARVIN, H. |
分类号 |
C12Q1/68;C07H1/00;C07H19/06;C07H19/067;C07H19/073;C07H19/10;C07H19/16;C07H19/167;C07H19/173;C07H19/20;C07H21/00;C07H21/02;C07H21/04;(IPC1-7):C07H21/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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