DEVICE FOR THE MULTIPLICATION AND DETECTION OF NUCLEIC ACIDS

摘要

The invention relates to a device for the multiplication and detection of nucleic acids, comprising a temperature controller and/or regulation unit, a reaction chamber, containing a substrate with a detection surface on which a substance library is immobilised and an optical system, by means of which the temporal process of formation of deposit on the detection surface may be detected. The temperature in the reaction chamber may be controlled and/or regulated by means of the temperature controller and/or regulation unit. The invention further relates to a method for the amplification and qualitative and quantitative detection of nucleic acids in a sample, comprising the following steps: a) introduction of the sample into a reaction chamber, formed by a capillary gap between a chamber support and a microarray, whereby the microarray comprises a substrate with nucleic acid probes arranged on array elements thereon, b) amplification of the nucleic acid for detection in the reaction chamber, by means of a cyclical amplification reaction, c) detection of a hybridisation between the nucleic acid for detection and the nucleic acid probes immobilised on the substrate without removal from the reaction chamber of molecules which are not hybridized with the nucleic acids immobilised on the substrate.