DETECTION OF BACTERIAL KIDNEY DISEASE

摘要

A real-time PCR method for detecting and quantifying the presence or absence of Renibacterium salmoninarum, the causative organism of Bacterial Kidney Disease, in a sample is disclosed. The method includes the steps of combining with a sample a pair of PCR primers that bind to a DNA that is specific for the organism and a labeled probe that also binds to the DNA, and performing one or more rounds of PCR on the sample. The probe undergoes a change with each round of amplification of the DNA during the PCR reaction, which change causes a change in the signal provided by the label, thus providing a detection of the DNA and, if desired, a quantification, of the level of the DNA, and thus the level of the organism, in the sample. Figure 1 is a diagrammatic representation showing the placement of the preferred primers and probe of the invention on the p57 gene in relation to the primers used for nested PCR.