发明名称 |
Method of determining nucleic acid base sequence |
摘要 |
A method of determining the base sequence of a nucleic acid characterized by involving: the step of amplifying a template nucleic acid in the presence of at least two primers each having a tag sequence, a primer specific to the template nucleic acid and a DNA polymerase, wherein the primers having tag sequences have each the tag sequence in the 5'-terminal side thereof and a specific base sequence consisting of three or more nucleotides in the 3'-terminal side; and the step of directly sequencing the amplified fragments obtained in the above step.
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申请公布号 |
US2004203008(A1) |
申请公布日期 |
2004.10.14 |
申请号 |
US20030415487 |
申请日期 |
2003.04.30 |
申请人 |
UEMORI TAKASHI;YAMASHITA HIROSHIGE;HOKAZONO SHIGEKAZU;SATO YOSHIMI;MUKAI HIROYUKI;ASADA KIYOZO;KATO IKUNOSHIN |
发明人 |
UEMORI TAKASHI;YAMASHITA HIROSHIGE;HOKAZONO SHIGEKAZU;SATO YOSHIMI;MUKAI HIROYUKI;ASADA KIYOZO;KATO IKUNOSHIN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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