<p>A tool that can screen bacteria for beta-lactamases against a panel of various antibiotics is desirable. A biosensor incorporating an indicator molecule into beta-lactamases may achieve this purpose, but it requires that the attached indicator molecule must not impair the binding affinity of the protein to a great extent to provide a higher sensitivity. A modified beta-lactamases with a residue on the ohm-loop or outside the ohm-loop but close to the active site of beta-lactamase being replaced by a reactive residue is developed in this invention.</p>
申请公布号
WO2004087925(A1)
申请公布日期
2004.10.14
申请号
WO2004CN00253
申请日期
2004.03.25
申请人
THE HONG KONG POLYTECHNIC UNIVERSITY;WONG, KWOKYIN;LEUNG, THOMAS, YUNCHUNG;CHAN, PAKHO