摘要 |
PURPOSE: To directly multiply an objective DNA without passing through a process for separating and purifying the nucleic acid, by coagulating the blood ingredients of a specimen containing blood as a main component, and subsequently subjecting the coagulated blood ingredients to a PCR. CONSTITUTION: The method for synthesizing the nucleic acid comprises coagulating blood ingredients in a specimen containing blood as a main component by a thermal treatment method, etc., and subsequently subjecting the coagulated blood ingredients to a polymerase chain reaction(PCR) for multiplying the base sequence of the objective DNA. The nucleic acid can simply and rapidly be synthesized from a specimen containing a large amount of blood in a state free from contamination. |