发明名称 |
METHOD FOR ENHANCING AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION BY PRE-TREATMENT OF SUGAR OR SALTS, IN WHICH THE METHOD IS APPLIED TO TRANSFORMATION-HARD PLANTS |
摘要 |
PURPOSE: A method for enhancing Agrobacterium-mediated genetic transformation by pre-treatment of sugar or salts is provided, thereby obtaining high efficiency of transgenic plants, so that it can be applied to transformation-hard plants. CONSTITUTION: The method for enhancing Agrobacterium-mediated genetic transformation by pre-treatment of sugar or salts comprises the steps of: inducing embryogenic callus by culturing zygotic embryo of ginseng and Cortex Acanthopanacis; preparing Agrobacterium and plasmid for genetic transformation; dipping embryogenic callus and cotyledon in 0.1M to 2M sucrose solution for 1 to 12 hours or dipping embryogenic callus and cotyledon in 0.01M to 0.2M of magnesium sulfate for 1 to 60 minutes; co-culturing the pretreated embryogenic callus and cotyledon with Agrobacterium and selecting transformed embryo using antibiotics such as kanamycin; and analyzing the gene insertion and expression by using Gus reaction.
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申请公布号 |
KR20040086974(A) |
申请公布日期 |
2004.10.13 |
申请号 |
KR20030021171 |
申请日期 |
2003.04.03 |
申请人 |
CHOI, YONG EUI |
发明人 |
CHOI, YONG EUI;JUNG, JAE HUN |
分类号 |
A01H4/00;(IPC1-7):A01H4/00 |
主分类号 |
A01H4/00 |
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