ARCTII FRUCTUS EXTRACT WITH INHIBITION EFFECT ON THE PRODUCTION OF INTERLEUKIN-1βCONVERTING ENZYME

摘要

PURPOSE: Provided is an Arctii Fructus extract with inhibition effect on the production of interleukin-1β converting enzyme. The Arctii Fructus extract is thus used for the prevention and treatment of inflammatory diseases caused by the increase of interleukin-1β converting enzyme. CONSTITUTION: A therapeutic for inflammatory diseases comprises the extract of Arctii Fructus as an active ingredient. The extract contains at least one active ingredient selected from compounds represented by the formulae(1,2,3) and their pharmaceutically acceptable salts. A method for isolating the active ingredient from Arctii Fructus comprises the steps of: extracting Arctii Fructus with methanol, followed by concentration under reduced pressure and extraction with ethylacetate to give ethylacetate extract; concentrating the ethylacetate extract, followed by dissolution in a trace amount of the mixed solvent, performing chromatography and concentration of active fractions to give concentrated solution; dissolving the concentrated solution in methanol and performing chromatography to collect active fractions; and separating the active fractions by chromatography, followed by removing the solvent with a vacuum dryer and freeze-drying to give compounds of the formulae(1,2,3).