Method of inactivating ribonucleases at high temperature

摘要

Methods for protecting RNA from RNase degradation and for inactivating RNases in solution are disclosed. The invention includes methods for protecting RNA during storage, for performing quantitative PCR reactions, or for preparing cDNA. The method includes using a combination of an RNase inhibitor in a solution containing or devoid of reducing agents and high heat to render RNases inactive.