| 摘要 |
PURPOSE: A method for production of a complex-specific egg yolk(IgY) antibody containing various antibodies including anti-helicobacter pylori IgY, anti-salmonella enteritidis IgY and anti-staphylococcus aureus IgY is provided. Therefore, functional eggs containing water-soluble complex-specific IgY are produced to increase human health and develop health foods industry. CONSTITUTION: The method for production of a complex-specific egg yolk antibody(IgY) containing various antibodies comprises the steps of: preparing antigens including Helicobacter pylori antigen, Helicobacter pylori urease antigen, r-glutamyltranspeptidase antigen, vacuolating cytotoxin(VacA) antigen, catalase antigen, Salmonella enteritidis antigen, Staphylococcus aureus antigen, Staphylococcus aureus exotoxin antigen, and ETEC(enterotoxigenic E. coli) exotoxin antigen; administering the antigens into a 19 week-old laying hen, a 21 week-old laying hen and a 25 week-old laying hen, sequentially; and isolating IgY using ionic water of pH 3 to 6 or pH 9 to 11 with one or more of glucose, maltose, sucrose, sorbitol, trehalose and mannitol. |
