发明名称 |
RNA POLYMERASE TRANSCRIPTION PROMOTERS AND METHOD FOR DETERMINING BASE SEQUENCE |
摘要 |
An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof. <CHEM> A method of sequencing DNA in which nucleic acid transcripts are obtained using an RNA polymerase and a DNA fragment as a template, the resulted nucleic acid transcripts are separated, the nucleic acid sequence is determined from the separated fractions wherein the nucleic acid transcription reaction is carried out in the presence of a compound selected from a group of compounds represented by the above formula (I). The polyamine compounds above have outstanding accelerating activity on transcription activity of RNA polymerase. Therefore, use of the polyamine compounds in a DNA sequencing method using RNA polymerase can make a length of DNA sequence that can be determined in one sequencing longer. |
申请公布号 |
EP1061130(A4) |
申请公布日期 |
2004.10.06 |
申请号 |
EP19990959936 |
申请日期 |
1999.12.21 |
申请人 |
RIKEN |
发明人 |
IWATA, MASAAKI RIKEN;HAYASHIZAKI, YOSHIHIDE RIKEN |
分类号 |
C12N15/09;C07C211/14;C07C311/18;C12N9/12;C12P19/34;C12Q1/68;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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