发明名称 |
Methods, compositions, and kits for mutation detection in mitochondrial DNA |
摘要 |
Methods, compositions, and kits for detecting mutations in the entire human mitochondrial genome. A preferred method includes amplifying mtDNA from a biological sample by polymerase chain reaction of total DNA using a plurality of pre-selected primer pairs to generate overlapping amplicons; cleaving the amplicons using restriction enzymes to produce fragments suitable for analysis by denaturing high performance liquid chromatography (DHPLC); denaturing and re-annealing the amplicons; and fragment analysis by DHPLC to detect the presence or absence of heteroduplex molecules.
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申请公布号 |
US2004191769(A1) |
申请公布日期 |
2004.09.30 |
申请号 |
US20020202162 |
申请日期 |
2002.07.24 |
申请人 |
TRANSGENOMIC, INC. |
发明人 |
MARINO MICHAEL A.;MCANDREW PATRICIA |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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