发明名称 |
Three cube fret method (3-fret) for detecting fluorescence energy transfer |
摘要 |
The invention provides a method for determining a measure of FRET comprising obtaining sequential fluorescent intensity readings from a specimen, such as a cell using three filter cubes. Simple equations manipulate readings from each of the filter sets or cubes to specify a unitless index of FRET called the FRET ratio (FR). FR bears a linear relation to FRET efficiency E. The method also provides for determining the fraction of acceptor-tagged molecules bound by donor-tagged molecules; the relative affinity of binding; and the strength of FRET interactions when all acceptor-tagged molecules are bound by donor. The latter determination enables estimates of the physical distance and/or orientation between interacting fluorophore molecules. The method can be used to detect analytes or inter- or intramolecular interactions. In a preferred aspect, the method is used in an HTS assay to identify modulators of such interactions.
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申请公布号 |
US2004191786(A1) |
申请公布日期 |
2004.09.30 |
申请号 |
US20030467964 |
申请日期 |
2003.12.18 |
申请人 |
YUE DAVID T.;ERICKSON MICHAEL G. |
发明人 |
YUE DAVID T.;ERICKSON MICHAEL G. |
分类号 |
C12Q1/68;G01N33/48;G01N33/50;G01N33/53;G01N33/542;G06F19/00;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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