| 摘要 |
A strategy for semiautomatic sequencing of argentinated (silver-containing) oligopeptides is described. The method of sequencing described is based on a search algorithm that identifies a triplet peak relationship in a product ion spectrum of the [M+Ag]<+ >ion of an oligopeptide. The ions that constitute a triplet are [bn+OH+Ag]<+>, [bn-H+Ag]<+>, and [an-H+Ag]<+>, which are separated by 18 and 28 m/z units, respectively. The difference in the m/z values of adjacent triplets identifies the residue that is "cleaved". Observation of the [yn+H+Ag]<+ >ion containing the cleaved residue confirms the assignment.
|
