Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction

摘要

A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5'-3'-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.