| 摘要 |
FIELD: biotechnology, preparative biochemistry. ^ SUBSTANCE: invention can be used in medicine, cosmetology, dermatology, biochemistry and food industry and in investigation aims also. Method for preparing the collagenase preparation involves homogenization of the parent collagen-containing raw, separation of extract by centrifugation, chromatography purification on ion-exchange resin and the following elution of active fraction, dialysis of eluate and lyophilic drying the final preparation. As the parent collagenase-containing raw method involves using skin beetle larvae of genus Dermestes. Homogenization is carried out in sodium chloride solution with addition of sodium azide in the volume ratio of biomass to solution = 1:(2-2.5). Chromatography purification is carried out on DEAE-Sepharose column equilibrated with MES-buffer with addition of 0.005 M of calcium chloride and elution is carried out with Tris-buffer with addition of sodium chloride, calcium chloride and ethyl alcohol. Invention provides high yield of collagenase, enhanced specific activity of enzyme by 5-10 times and reduces the process time for preparing. ^ EFFECT: improved preparing method. ^ 4 dwg, 2 ex |
