| 摘要 |
FIELD: microbiology, immunology. ^ SUBSTANCE: invention can be used for establishment of etiological significance of isolated strains of microorganisms. Method is carried out by growing analyzed culture of microorganisms in solid nutrient medium, preparing bacterial suspension from the grown agar culture and its mixing with purified immunoglobulin. Control sample is prepared in parallel from buffered physiological solution and purified immunoglobulin. Experimental and control samples are incubated for 18-24 h, supernatant is separated from bacterial cells by centrifugation and the concentration of residual immunoglobulin is determined in experimental and control sample by immunoenzyme analysis. Anti-immunoglobulin activity is calculated by equation: AIgA = (Ck - Co)/Ck x 100% wherein AIgA is anti-immunoglobulin activity, %; Ck - concentration of residual immunoglobulin in control, mcg/ml; Co - concentration of residual immunoglobulin in experiment, mcg/ml. Method allows enhancing the effectiveness of assay of anti-immunoglobulin activity of microorganisms due to elevating sensitivity of method and enhancing precision of quantitative estimation of anti-immunoglobulin activity. ^ EFFECT: improved method for assay. ^ 4 tbl, 2 ex |
