Intra-retinal pH mapping involves forming two fluorescent images in the form of two two-dimensional matrices of pixels, and, for each pixel, calculating the ratio between fluorescent levels in response to the excitation wavelengths

摘要

The method involves illuminating the retina successively with first and second (different) excitation radiation. Each radiation has a narrow spectrum centered on a wavelength belonging to the excitation spectra of each of two fluorescent markers applied to the retina. Respective fluorescent images in the form of two two-dimensional matrices of pixels, at the peak wavelengths of the fluorescence emission spectra, are formed. For each pixel the ratio between the fluorescence levels emitted in response to the two excitation wavelengths is representative of the pH of the retina, and a map of the pH of the retina is deduced. Independent claims are given for: (a) a device for intra-retinal pH mapping; and (b) a device for photocoagulation of regions of the retina comprising the intra-retinal pH mapping device and a laser.