EXPRESSION CASSETTE OF RECOMBINATION PROTEIN FOR EFFICIENT PRODUCTION OF PROTEASE USING COLICIN PROMOTER TO MASS-PRODUCE THE PROTEASE BY ONE PURIFICATION PROCESS, AND IMPROVE PRODUCTIVITY THEREOF

摘要

PURPOSE: An expression cassette of a recombination protein for the efficient production of a protease using a colicin promoter is provided, thereby easily mass-producing the protease by one purification process, and improving productivity of the protease. CONSTITUTION: The expression cassette of a recombination protein for the efficient production of a protease using a colicin promoter comprises the steps of: digesting an expression plasmid containing a transcription termination factor with enzyme, inserting the digested DNA fragments into a plasmid pLitmus28 to modify the restriction enzyme recognition site, and inserting the modified digested DNA fragments of the transcription termination factor into a colicin plasmid; inserting a gene fragment for affinity purification into the colicin plasmid; and inserting a gene Tag of the protease into the colicin plasmid.