METHOD FOR PRODUCING MANNOSYL ERYTHRITOL LIPID

摘要

PROBLEM TO BE SOLVED: To provide a method for producing mannosyl erythritol lipids as a kind of biosurfactant in improved efficiency( in greater quantities, at higher speed and in higher yield ). SOLUTION: The method comprises culturing microorganisms having the ability to produce the mannosyl erythritol lipids in a culture fluid containing vegetable fat-and-oil and nutrients while adjusting the pH of the culture fluid to 5.2-5.8. Preferable embodiments include the following: The culture is carried out at 10-20 mass% in the initial concentration of the vegetable fat-and-oil. The culture is started at 14-20 mass% in the initial concentration of the vegetable fat-and-oil and additional vegetable fat-and-oil is fed at a rate of 0.9-1.1 g/L/h from 6-7 days after starting the culture into the culture fluid. The culture is carried out under the condition of being≥25 mass% in the total concentration of the vegetable fat-and-oil( initial plus additional ). COPYRIGHT: (C)2004,JPO&NCIPI