发明名称 |
A METHOD OF PREPARING LIGANDS FOR HYDROPHOBIC INTERACTION CHROMATOGRAPHY |
摘要 |
<p>The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.</p> |
申请公布号 |
WO2004078311(A1) |
申请公布日期 |
2004.09.16 |
申请号 |
WO2004SE00316 |
申请日期 |
2004.03.05 |
申请人 |
AMERSHAM BIOSCIENCES AB;JOHANSSON, BO-LENNART;MALOISEL, JEAN-LUC;THEVENIN, NICOLAS |
发明人 |
JOHANSSON, BO-LENNART;MALOISEL, JEAN-LUC;THEVENIN, NICOLAS |
分类号 |
B01D15/32;B01J20/286;(IPC1-7):B01D15/08;B01J20/30;B01J20/22 |
主分类号 |
B01D15/32 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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