ARTIFICIAL ANTIBODY LIBRARY WITH SUPER-REPERTORY

摘要

A library of artificial antibodies with a ultra-wide repertory, whose titer is over 10<11> or more, is obtained by amplifying gene fragments including the CDR1 and CDR2 regions and gene fragments including the CDR3 region of the VH or VL region in an immunoglobulin gene by PCR using a cDNA library as a template, inserting the VH and VL libraries which have a small amount of contamination of a repertory which cannot be expressed and are highly stable into non-expression vectors, and shuffling the regions between the VH region in the VH library and the VL region in the VL library by introducing the libraries into a host.