| 摘要 |
Described herein is a eukaryotic cell that is stably transfected with a polycistronic vector for expressing functional antibodies, the vector comprises a polycistronic transcription system comprising the following elements operably linked in the 5' to 3' orientation: (i) a promoter operable in a eukaryotic cell; (ii) a first cistron comprising a first DNA sequence comprising a 5' start codon, a nucleotide sequence encoding an antibody light chain, and a 3' terminal stop codon, wherein said first DNA sequence does not comprise a poly A sequence at its 3' end; (iii) an internal ribosome entry site (IRES) obtained from a member selected from the group consisting of a cardiovirus, a herpes virus and a poliovirus; (iv) at least an additional cistron comprising a second DNA sequence comprising a 5' start codon, a nucleotide sequence encoding an antibody heavy chain, and a 3' terminal stop codon, wherein said second DNA sequence comprises a poly A sequence at its 3' terminus; wherein the IRES is operably linked to the 5' end of the additional cistron with no intervening regulatory elements; and wherein the functional antibodies expressed by the polycistronic vector specifically bind to a tumor associated antigen. Further described is a method of producing functional antibodies comprising culturing the eukaryotic cell of the invention in a cell culture to produce functional antibodies. |
