发明名称 |
Method for selecting tumours expressing HLA-G, sensitive to anticancer treatment and uses |
摘要 |
The invention concerns a method for selecting tumours expressing HLA-G, sensitive to an anticancer treatment, which inhibits or prevents the HLA-G activity of said tumours and the uses thereof. Said method enable to establish either the HLA-G, transcription profile of a solid tumour or the HLA-G expression profile of a solid tumour. The method for establishing the HLA-G transcription profile consists in: (i) drawing a tumoral sample; (ii) extracting the mRNA; (iii) reverse transcription (RT) of said RNA: (iv) successive or simultaneous amplification of the cDNA's obtained in (iii) in the presence of primers specific to each HLA-G isoform and analysing the resulting amplification products, by electrophoresis and/or specific hybridisation and (v) establishing said sample HLA-G transcription profile. The method for establishing the HLA-G expression profile consists in: (i) drawing a tumoral sample; (ii) optionally marking said sample cells; (iii) carrying out a lysis of the cells; (iv) contacting said cells which have been subjected to lysis with different antibodies directed against the class I HLA-G antigens, to form, optionally HLA-G isform/antibodies complexes; and (v) establishing said sample HLA-G expression profile by detecting the complexes formed in step (iv).
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申请公布号 |
US6790638(B1) |
申请公布日期 |
2004.09.14 |
申请号 |
US20000622583 |
申请日期 |
2000.10.13 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE |
发明人 |
CAROSELLA EDGARDO DELFINO;DAUSSET JEAN;MOREAU PHILIPPE;PAUL PASCALE;ROUAS-FREISS NATHALIE |
分类号 |
A61K31/7088;A61K39/00;A61K45/00;A61P35/00;C07K14/705;C07K16/28;C12N15/09;C12Q1/02;C12Q1/68;G01N33/574;(IPC1-7):C12P21/06;C01N33/52;C07K1/00 |
主分类号 |
A61K31/7088 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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