The present invention involves a method of coupling disulfide bridge containing proteins to a carrier by inducing the formation of thiol groups on a protein with irradiation, and coupling the protein to the carrier. The formation of thiol group(s) in the protein is a consequence of the disruption of disulfide bridges following electronic excitation of aromatic amino acid residues located in close spacial proximity to the disulfide bridge. The light-induced coupling method facilitates the orientated immobilization of a protein on a carrier.
申请公布号
WO2004065928(A3)
申请公布日期
2004.09.10
申请号
WO2004DK00047
申请日期
2004.01.22
申请人
BIONANOPHOTONICS A/S;PETERSEN, STEFFEN, BJOERN;DA CRUZ AUGUSTO NEVES PETERSEN, MARIA, TERESA
发明人
PETERSEN, STEFFEN, BJOERN;DA CRUZ AUGUSTO NEVES PETERSEN, MARIA, TERESA