ESTABLISHMENT OF DISEASE NERVE CELLS BY COMBINING METHOD OF HIGHLY EFFICIENTLY INDUCING NERVE DIFFERENTIATION WITH DISEASE MUTATION KNOCKIN ES CELLS

摘要

It is intended to provide nerve degenerative disease neurons within a short time by differentiating disease mutation knockin ES cells by a highly efficient differentiation method. A knockin ES cell having a gene mutation, which is causative of Alzheimer's disease (i.e., a nerve degenerative disease), is differentiated by a novel highly efficient differentiation method to thereby establish Alzheimer's disease model neurons. Thus, a knockin ES cell having a gene mutation causative of Alzheimer's disease is established. Further, the ES cell is differentiated by the novel highly efficient differentiation method and thus Alzheimer's disease model neurons expressing a neuron marker at a ratio of about 90% or higher can be established. Furthermore, a disease gene mutation knockin animal is constructed and thus a disease model animal, in particular, an Alzheimer's disease model is provided.