DNA AND AMINO ACID SEQUENCES OF A TYROSINE AMMONIA LYASE ENZYME FROM THE BACTERIUM RHODOBACTER SPHAEROIDES

摘要

A novel tyrosine ammonia lyase enzyme was identified in the bacterium Rhodobacter sphaeroides. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was cloned by direct amplification using the genomic DNA and was expressed in E. coli.