SEQUENCE SPECIFIC DNA RECOMBINATION IN EUKARYOTIC CELL

摘要

PROBLEM TO BE SOLVED: To provide a simple and controllable recombination system and a practical means for carrying out stable targeted integration of a desired DNA sequence and to provide a method for affording an improved protein expression system.

SOLUTION: The method for sequence specific recombination of a DNA in a eukaryotic cell is provided. The method comprises a step of introducing a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell and a step of performing the sequence specific recombination by a bacteriophage lambda integrase Int.

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