发明名称 |
METHYLOTROPHIC BACTERIUM FOR THE PRODUCTION OF RECOMBINANT PROTEINS AND OTHER PRODUCTS |
摘要 |
<p>The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing am expression vector into the methylotrophic bacterium, the expression vector comprising a polynucleotide sequence, coding for a peptide or protein, or allowing for production of a product from metabolic engineering under the control of a regulated promoter. the method also comprises growing the genectically modified methylotrophic bacterium in a minimal salts medium lacking organic sugars and containing methanol. A metal ion may be used for regulating the expression of the polynucleotide sequence by the promoter.</p> |
申请公布号 |
EP1453981(A1) |
申请公布日期 |
2004.09.08 |
申请号 |
EP20020781026 |
申请日期 |
2002.11.29 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
MIGUEZ, CARLOS, B.;FIGUEIRA, MARIANNE, M.;LARAM E, LOUISE;MURRELL, COLIN, J. |
分类号 |
C12N15/74;C12P21/02;(IPC1-7):C12R1/01;C12N1/32 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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