Method for preparing a cartilaginous neo-tissue

摘要

The invention concerns the preparation of a cartilaginous neo-tissue that is capable of being grafted. The method consists in: a) culturing chondrogenic cells, which are either autologous chondrocytes or chondrocyte precursor cells prepared in vitro from pluripotent stem cells; b) bringing said chondrogenic cells into contact with a chitosan hydrogel-preferably having a degree of acetylation in the range 40% to 60%-its amphiphilic properties and degree of acetylation being such that said cells adhere naturally to the outer surface of said hydrogel; c) covering the hydrogel/cell ensemble obtained with a culture medium; and d) allowing a cartilaginous neo-tissue to develop in contact with the chitosan hydrogel for a minimum period of two weeks, frequently renewing the culture medium.