MINIMALLY IMMUNOGENIC VARIANTS OF HUMANIZED COL-1 ANTIBODY AGAINST CARCINOEMBRYONIC ANTIGEN

摘要

The present disclosure provides humanized COL-1 monoclonal antibodies that are minimally immunogenic and that retain carcinoembryonic antigen binding affinity. In one embodiment, a humanized COL-1 antibody includes an amino acid substitution at position 61 in a heavy chain complementarity determining region 2 of the murine COL-1 antibody. In another embodiment, a humanized COL-1 antibody includes an amino acid substitution at position 24, 25, and 27 in a light chain complementarity determining region 1 of the murine COL-1 antibody. In several embodiments, methods are disclosed for the use of a humanized COL-1 antibody in the detection or treatment of a tumor in a subject. Also disclosed is a kit including the humanized COL-1 antibody described herein.