| 摘要 |
<P>PROBLEM TO BE SOLVED: To obtain a plasmid plastid vector useful for gene transfer to a bacterium of the genus Methylophilus. <P>SOLUTION: An autonomously replicating sequence functioning by a bacterium of the genus is isolated by transforming the bacterium of the Methylophilus with a ring-shaped DNA containing a chromosome DNA fragment of the bacterium of the genus Methylophilus and a DNA fragment having a marker gene functioning in the cells of the bacterium, selecting a transformant expressing the marker gene from transformants and isolating the ring-shaped DNA from the selected transformant. <P>COPYRIGHT: (C)2004,JPO&NCIPI |
