USE OF CASPASE ENZYMES FOR MATURATION OF ENGINEERED RECOMBINANT POLYPEPTIDE FUSIONS

摘要

The invention relates to methods of producing and isolating recombinant proteins using fusion protein constructs comprising specific recognition sit es for a maturating protease. The invention specifically relates to the use of caspase proteases for the maturation of engineered recombinant fusion protei ns or polypeptides. These molecules are engineered using recombinant DNA technology to comprise a specific target sequence for a caspase between a first fusion part and the polypeptide of interest. After processing, the desired mature format of the protein or polypeptide is obtained.