NOVEL METHOD FOR DETECTING AND ANALYZING PROTEIN INTERACTIONS IN VIVO

摘要

The invention relates to a method for detecting and analyzing protein interactions in a cell, comprising the following steps: a) providing the activity of at least one enzyme selected from the group consisting of recombinases and proteases in the cell following a protein interaction; b)continued generation of an active reporter protein in the respective cell following the enzyme activity from step a) for a period of time exceeding that of the protein interaction from step a), and; c) producing a detection signal using the reporter proteins generated in b). The invention also relates to reverse configurations of the above method for detecting and analyzing protein interactions in a cell, whereby following the induced dissociation of a defined interaction between proteins, the activity of at least one enzyme selected from the group consisting of recombinases and proteases is provided in the cell and is converted into a permanent detection signal of the cell. The invention additionally relates to cells that express the inventive protein constituents, and to kits that provide the inventive protein constituents at the DNA level in the form of suitable expression vectors and, optionally, suitable transfectable or infectable cells. The provided cells can optionally express individual inventive protein constituents in a stable or transient manner.